Unfed female parasites had a type i survivorship curve and lived an average of 23. Larval blood cells of drosophila melanogaster stained with fluorescently tagged phalloidin, in green, to distinguish cortical actin at the cell boundary, and dapi, in blue, to identify the nucleus engulfing red fluorescent escherichia coli. Mrb was first discovered in the texas rice belt in 1988 and soon received attention from producer organizations and support industries. The sugarcane borer, diatraea saccharalis fabricius, is native to the western hemisphere, but not. This wasp was imported from the united states, aiming to control pseudaulacaspis pentagona targionitozzetti hemiptera. No matter which continent, ecosystem, or type of crop culture, a field of rice is usually infested by more than one stem borer species. Our results showed that after nine hours of herbivory, sugarcane genes were upregulated and nine were. Early biological control attempts ofdiatraea saccharalis in barbados had failed. Here we present an overview of sugarcane stem borer biology and. Diatraea lineolata and diatraea saccharalis lepidoptera.
The sex ratios and number of parasite progeny were. Genetic diversity analysis with rapd linked to sex. Crambidae are moths with stemboring larvae that feed and develop on economically important grasses. Structural characteristics and phylogenetic analysis of. F2 screen for resistance to a bacillus thuringiensismaize.
Files are available under licenses specified on their description page. Electrophysiological studies and identification of possible. Laboratory rearing and biology of the parasite cotesia. Diatraea saccharalis is the principal pest of sugarcane in brazil and is found throughout. Subsequent intensive release campaigns of several parasite species from the neotropics. Larvae completed development at temperatures ranging from 22 to 34c. Biology and technology a n i n t e r n a t i o n a l j o u r n a l sequence and analysis of the mitochondrial dna control region in the sugarcane borer diatraea saccharalis lepidoptera. Our aim was to contribute to the knowledge of the biology of d. Department of biochemistry, school of science, university of oriental.
All structured data from the file and property namespaces is available under the creative commons cc0 license. Defining terms for proactive management of resistance to bt. Diatraea saccharalis is an insect that causes considerable losses in the sugar cane crop. World bibliography of rice stem borers stem borers are ubiquitous pests of rice worldwide figs. Currently, the efficacy of dna barcoding is being assessed using tools established by the cbol, as outlined in the barcode of life data systems bold, 2008, website. Pdf the control of the major sugarcane pest, diatraea saccharalis, is limited by the stem location of the caterpillar. Therefore, a highly efficient control of diatraea saccharalis is imperative especially if the 6 million. This article focuses on the history of pest management as it relates to the control of this stem borer in louisiana sugarcane, and how control practices have become more in tune with integrated pest management paradigms. Crambidae juliana pereira bravo, joice felipes, daniela bertolini zanatta, jose luis da conceicao silva and maria aparecida fernandez.
Apr 01, 2014 the other three cases are fieldevolved resistance to cry1ac in bt cotton by pectinophora gossypiella saunders in china wan et al. Development of diatraea saccharalis lep pyralidae at. A novel f 2 screening technique was developed for detecting resistance in sugarcane borer, diatraea saccharalis f. This assay was used to evaluate the effects of the gene s6kinase on the phagocytic ability of blood cells. Regulation of the larval transcriptome of diatraea saccharalis. Control of scsb is very difficult and expensive due to the typical feeding behavior of the larvae into the sugarcane stem. Jose roberto postali parra, one of the authors of this article. In this application a key role is played by the concept of controllability, which means that it is possible to steer the system to an equilibrium in given time. Progress in the chemistry of organic natural products, volume 82 edited by w. Sugarcane stem borers of the colombian cauca river. In the southern usa the sugarcane borer, diatraea saccharalis, and the rice stalk borer, chilo plejadellus are common stem borers attacking rice. The aim of this work was to assess the activity of neem trade name. Status, biology, and control sugarcane stem borers of the.
A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway. This study investigated whether these moths have diverged from a native host plant, corn, onto introduced crop plants including sorghum, sugarcane, and rice. Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. Parasites accepted and used third to sixthinstar diatraea saccharalis f. The sugarcane borer moth, diatraea saccharalis, is one of the most important pests of sugarcane and maize crops in the western hemisphere. Diatraea saccharalis f is considered the most important pest of sugarcane in the united states. Electrophysiological studies and identification of possible sex pheromone components of brazilian populations of the sugarcane borer, diatraea saccharalis. Induction of differentiation in acute promyelocytic leukemia cells hl60 by the verticillin derivative sch 52900. Insects free fulltext withinhost competition between. Africa and india resulted in the temporary establishment ofmetagonistylum minense andtrichogramma japonicum and the permanent establishment oflixophaga diatraeae andapanteles flavipes. Sugarcane stem borers of the colombian cauca river valley. Costeffective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field.
In order to test these products, an artificial diet for diatraea saccharalis. The insects infest rice crops throughout their growth, from the seedling stage to maturity. Reviewdna barcode information for the sugar cane moth borer diatraea saccharalis. Eriococcidae in the country of georgia and massachusetts usa, parts of its native and invaded ranges, on two species of beech. Pfti showed significant inhibitory activity against trypsinlike proteases present in the larval midguts on a. Institute facilities include seven laboratories equipped with following. The larvae, similarly to diatraea saccharalis, present a dark brown head, long bristles, and. Extension courses on insect rearing and nutrition techniques aiming biological control programs have been offered since 1980 annually or every 2 yr in different parts of the country, under the coordination of prof. Male adult insects of diatraea saccharalis n 38, d. Diatraea saccharalis history of colonization in the americas.
Genetic variability and gene flow among brazilian. Diatraea saccharalis an overview sciencedirect topics. There is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. Diatraea saccharalis, the sugar cane borer, is the major pest of sugar cane crops, and its caterpillarfeeding behavior, inside the stems, hampers control. Dna barcode information for the sugar cane moth borer. Sequence and analysis of the mitochondrial dna control region. Feb 22, 2011 braga dpv, arrigoni edb, burnquist wl, silvafilho mc, ulian ec 2001 a new approach for control of diatraea saccharalis lepidoptera. Adult sugarcane borer, diatraea saccharalis fabricius. Comparative densities of beech scale, cryptococcus fagisuga, hemiptera. Sugarcane stem borers of the genus of diatraea lepidoptera. A twoagent model applied to the biological control of the. Induced resistance to diatraea saccharalis lepidoptera.
Pdf a brazilian bacillus thuringiensis strain highly active to. Pdf genetic diversity and structure of brazilian populations of. Cooperation arrangements with developing countries. Binding analysis of bacillus thuringiensis cry1 proteins in. We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer diatraea saccharalis fabricius lepidoptera. Institute of biomedical sciences, federal university of rio grande do sul.
In order to test these products, an artificial diet for diatraea saccharalis fabricius, 1794. It was described by johan christian fabricius in 1794. Biology of sugarcane borer is described by holloway et al. As a result, the panicle is unfilled and whitish in color, rather than filled with grain and brownish in color. Biocarbo, itabirito, brazil at 10 mll 1% and 20 mll 2% contents on egg. The results showed that cry1ab was the most active, followed by cry1ac. Your use of this pdf, the bioone complete website, and all posted and associated content. Management of rice insect pests radcliffes ipm world textbook. Various pest management strategies are employed against d. Ijms free fulltext sugarcane serine peptidase inhibitors. Pathogens associated with sugarcane borers, diatraea spp.
Purification and characterization of a trypsin inhibitor from. Crambidae is a pest that directly damages the plant by boring into the stalk. Diatraea saccharalis, the sugarcane borer, is a species of moth of the family crambidae. Crambidae form a species complex that causes serious economic losses to sugarcane production in the cauca river valley and other regions of colombia. It is native to the caribbean, central america, and the warmer parts of south america south to northern argentina. The aim of the present study was to analyze the susceptibility and binding interactions of three cry1a proteins and cry1fa in a brazilian d. The first insect introduced in brazil as a biological control bc agent to control agricultural pests was encarsia berlesei howard hymenoptera. Sugarcane borer, diatraea saccharalis fabricius insecta. Before sharing sensitive information online, make sure youre on a. In our model system, we highlight the antagonistic effects of the coinoculation of beauveria bassiana and. Pdf the biology of diatraea flavipennella lepidoptera.
Base fertil, chapadao do sul, brazil and pyroligneous extracts trade name. Crambidae herbivory was investigated using a macroarray spotted with 248 sugarcane expressed sequence tags ests encoding serine peptidase inhibitors, serine peptidases. Crambidae through the expression of an insecticidal cryiab protein in transgenic sugarcane. Esterase3 polymorphism in the sugarcane borer diatraea saccharalis lepidoptera, pyralidae article pdf available in genetics and molecular biology 251 march 2002 with 35 reads. Deleterious activity of natural products on postures of. The sugarcane borer diatraea saccharalis fabricius, 1794 lepidoptera. The control of lepidoptera pests should be carried out before hatching of their caterpillars to avoid damage to the crops. Biology of the parasite cotesia flavipes cameron and rearing procedures appropriate for smallscale laboratory production of the parasite are described. This document is eeny217, one of a series of the entomology and nematology department, ufifas extension.
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